Stephen Lewis , Jan 06, 2011; 12:00 p.m.
If you have more than one object, or objects at differing distances from the film plane, you will need to pay particular attention to using the greatest depth of field you can (without introducing diffraction). You should also consider using a tripod with a focusing stage/rail to minimize shake, and/or using flash or a macro light. I personally prefer hand held, but over time I'm finding in spite of lots of good muscular control, age is beginning to take its toll and I'm having to gracefully accept tools to improve my focus and sharpness. Another thing to consider is a cable release....using it means just one less source of vibration. Lastly, consider turning off everything auto (auto focus, auto exposure, etc.) and learning how to do things manually where you have complete control over the processes. Take a read in the learning tab at the top of the page under the macro section for more hints and reference sources.